Alx1 216285

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Gene ID: 216285
Gene symbol: alx1
Gene Description: alx homeobox 1
Aliases: AI314867; C130005I02; Cart1
Human Ortholog Gene ID: [8092]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Helix-Turn-Helix Group
DBD Protein Group/Family Classification: Helix-Turn-Helix Group / Homeodomain Family
Homolog Cluster(s): 103889

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[8756334] Transactivation Human Not Selected Reporter cotransfection assays describe it as a repressor. In the latter study, this repression was mediated by footprint II, an AT-rich element that functions as an inhibitory site of prolactin gene expression in nonpituitary cells and which was used to clone Cart-1. Tim Hughes
[9847249] DNA Binding Mouse Not Selected Cart1 is a homeodomain protein that binds preferentially to the palindromic repeats of the sequence 5'-TAAT-3' with a three base pair insertion (P3 element) as determined by gel-shift assay. Tim Hughes
[12390248] Transactivation Rat Not Selected Reporter cotransfection assays have yielded describes it as an activator. Tim Hughes
[9753625] DNA Binding; Transactivation Human Strong Binding site selection studies (CASTing) identified a consensus CART1 binding site. Sequence-specific binding was confirmed by gel shift assay using a bacterially expressed GST-CART1 fusion protein.

Transient transfection studies in HeLa cells show that CART1 activates transcription through the CASTing-selected and consensus binding sites.

Rob Sladek
[12390248] Transactivation Rat Strong Transactivation and nuclear localization domains were identified by transient transfections in HEK293 cells using a fusion protien containing Cart1 deletion mutants joined to the GAL4 DNA-binding domain.

DNA binding properties of the Cart1 homeodomain were demonstrated by gel shift assay using bacterially expressed GST fusion proteins.

Rob Sladek
Additional Reviewer Comments:
Reviewer: Rob Sladek

Binding site selection studies (CASTing) identified a consensus hCART1 binding site. Sequence-specific binding was confirmed by gel shift assay using a bacterially expressed GST-CART1 fusion protein. The DNA binding properties of the isolated rCart1 homeodomain were demonstrated by gel shift assay using bacterially expressed GST fusion proteins. Transient transfection studies in HeLa cells show that hCART1 activates transcription through the CASTing-selected and consensus binding sites. Cart1 transactivation and nuclear localization domains were identified by transient trasnfections in HEK293 cells using a fusion protien containing rCart1 deletion mutants joined to the GAL4 DNA-binding domain.

Reviewer: Tim Hughes

Cart1 is a homeodomain protein that binds preferentially to the palindromic repeats of the sequence 5'-TAAT-3' with a three base pair insertion (P3 element) (PMID: 9847249), as determined by gel-shift assay. Reporter cotransfection assays have yielded different results regarding whether Cart1 is a transcriptional activator or repressor, with (PMID: 12390248) describing it as an activator and (PMID: 8756334) describing it as a repressor. In the latter study, this repression was mediated by footprint II, an AT-rich element that functions as an inhibitory site of prolactin gene expression in nonpituitary cells and which was used to clone Cart-1.


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Facts about Alx1 216285RDF feed
Gene description alx homeobox 1  +
Gene id 216,285  +
Gene judgment TF Gene  +
Gene symbol alx1  +, AI314867  +, C130005I02  +, and Cart1  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 103,889  +
Protein family 1.1 Helix-Turn-Helix Group/2 Homeodomain Family  +
Protein group 1.1 Helix-Turn-Helix Group   +
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