Arid4a 238247

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Gene ID: 238247
Gene symbol: arid4a
Gene Description: at rich interactive domain 4a (rbp1 like)
Aliases: A630009N03; A630067N03Rik; MmRBBP1; Rbbp1
Human Ortholog Gene ID: [5926]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Regulatory Activity: heterochromatin interaction/binding;

Transcription Factor Binding: tf co-factor binding

Homolog Cluster(s): 238247

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[10321733] Other; TF PPI Human; Other Strong Arid4A is part of E2F/DNA complexes in addition of pRB or p130 (EMSA with monkey CV-1 nuclear extracts).

HA-tagged Arid4A represses transcription of a luciferase reporter construct driven by the E2F-1 promoter (in human C33A cells). Arid4A Induces growth arrest (Inhibition of CV-1 cells colony formation).

Elodie Portales-Casamar
[10490602] Other; TF PPI Human Strong pRB interacts with HDACs (co-IP of endogenous proteins in H1299 or 293T cells).

Arid4A interacts with HDACs (co-IP of over-expressed proteins in H1299 or 293T cells). Arid4A associates with all members of the RB family (pRB, p107, and p130) in H1299 cells but not in 293T cells (co-IP of endogenous proteins). Arid4A contains two independent transcriptional repression domains (gene reporter assay with GAL4 fusion proteins in CHO cells). Transcriptional repression by Arid4A is both dependent and independent of HDAC activity (assessment of the repression activity of the GAL4 fusion proteins with use of HDAC inhibitor trichostatin A (TSA)). Both Arid4A and the pocket of pRB repressed the expression of luciferase from the E2F1-luc reporter carrying a E2F-dependent promoter (overexpressed proteins, no GAL4 fusion).

Elodie Portales-Casamar
Additional Reviewer Comments:
Reviewer: Elodie Portales-Casamar

Arid4A plays a central role in the repression of E2F-dependent transcription via RB family members, inducing growth arrest. Arid4A serves as a bridging molecule to recruit HDACs but also provides a second HDAC-independent repression function.


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Facts about Arid4a 238247RDF feed
Gene description at rich interactive domain 4a (rbp1 like)  +
Gene id 238,247  +
Gene judgment TF Gene  +
Gene symbol arid4a  +, A630009N03  +, A630067N03Rik  +, MmRBBP1  +, and Rbbp1  +
Gene taxonomy Transcription Regulatory Activity: heterochromatin interaction/binding  +, and Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 238,247  +
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