Arid4b 94246

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Gene ID: 94246
Gene symbol: arid4b
Gene Description: at rich interactive domain 4b (rbp1 like)
Aliases: 5930400I17; 6330417L24Rik; 6720480E17Rik; 9330186M13; BCAA; BRCAA1; RBBP1L1; Rbp1l1; SAP180
Human Ortholog Gene ID: [51742]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Regulatory Activity: heterochromatin interaction/binding
Homolog Cluster(s): 238247

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[12724404] Co-activation; TF PPI Human; Unknown Medium Arid4b (referred to by its alias, SAP180, in this PMID) repressed reporter transcription in a cotransfection assay in which an expression vector for Arid4b fused to the GAL4 DNA-binding domain was transfected into HEK-293 cells along with a luciferase reporter vector. Addition of Histone Deacetylase (HDAC) inhibitor TSA had little to no effect on the transcriptional reporession by GAL-Arid4b.

Human Arid4b co-immunoprecipitated with human mSin3A, HDAC1, HDAC2, and mSDS3, all components of the mSin3A co-repressor complex in a co-immunoprecipitation assay in which each of the co-repressor complex components was immunoprecipitated from K562 nuclear extracts, and Arid4b was detected through Western blotting using anti-Arid4b.

Stuart Lithwick
Additional Reviewer Comments:
Reviewer: Stuart Lithwick

Human Arid4b (referred to as SAP180 in this PMID) is likely a transcriptional cofactor, which represses gene expression in a complex with a variety of other co-repressor proteins. Arid4b represses luciferase reporter expression when fused to the GAL4-DBD and cotransfected into HEK-293 cells along with a luciferase reporter vector. In addition, Arid4b interacts with each of the components of the mSIN3A corepressor complex as demonstrated by co-immunoprecipitation from K562 nuclear extracts. This suggests that transcriptional repression by Arid4b is achieved via the action of Histone Deacetylase (HDAC). However, the addition of HDAC inhibitor TSA to the cotransfection experiment did not impact upon the ability of Arid4b to repress expression suggesting that it may be by other means.


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Facts about Arid4b 94246RDF feed
Gene description at rich interactive domain 4b (rbp1 like)  +
Gene id 94,246  +
Gene judgment TF Gene  +
Gene symbol arid4b  +, 5930400I17  +, 6330417L24Rik  +, 6720480E17Rik  +, 9330186M13  +, BCAA  +, BRCAA1  +, RBBP1L1  +, Rbp1l1  +, and SAP180  +
Gene taxonomy Transcription Regulatory Activity: heterochromatin interaction/binding  +
Homolog cluster 238,247  +
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