Arid5b 71371

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Gene ID: 71371
Gene symbol: arid5b
Gene Description: at rich interactive domain 5b (mrf1 like)
Aliases: 4930580B11; 5430435G07Rik; AI467247; Desrt; Mrf2; Mrf2alpha; Mrf2beta
Human Ortholog Gene ID: [84159]
Reviewer Judgement: TF Gene Candidate
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Helix-Turn-Helix Group
DBD Protein Group/Family Classification: Helix-Turn-Helix Group / Arid Domain Family
Homolog Cluster(s): 71371

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[8649988] DNA Binding; Transactivation Human Medium The nuclear protein MRF-2 binds to multiple sites on the modulator located upstream of the human cytomegalovirus major immediate early gene enhancer. Competitive binding assays demonstrate that MRF requires the presence of multiple A+T stretches for binding to DNA, rather than binding to a specific DNA sequence. Mutations of these stretches in the modulator reduce the binding activity of MRF, as well as the repressing activity on the enhancer. Shannan HoSui
[10329386] DNA Binding Mouse Strong Binding interference and binding site selection assays indicated that Mrf-2 requires the core sequence AATA(C/T) for high affinity binding. Kinetic analyses of several selected sequences indicated that the core sequence alone is not sufficient for high affinity binding, however. Kinetic analyses were also performed using a series of synthetic oligonucleotides with single base analogues at each position in the core sequence. Shannan HoSui
Additional Reviewer Comments:
Reviewer: Shannan HoSui

Mrf-2 contains the ARID (AT-rich interaction domain) binding motif. Binding to AT-rich sequences has been demonstrated by gel mobility shift assays, NMR and selection assays. Repressor activity has been shown for the cytomegalovirus enhancer.


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Facts about Arid5b 71371RDF feed
Gene description at rich interactive domain 5b (mrf1 like)  +
Gene id 71,371  +
Gene judgment TF Gene Candidate  +
Gene symbol arid5b  +, 4930580B11  +, 5430435G07Rik  +, AI467247  +, Desrt  +, Mrf2  +, Mrf2alpha  +, and Mrf2beta  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 71,371  +
Protein family 1.1 Helix-Turn-Helix Group/109 Arid Domain Family  +
Protein group 1.1 Helix-Turn-Helix Group   +
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