Ascl1 17172

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Gene ID: 17172
Gene symbol: ascl1
Gene Description: achaete-scute complex homolog 1 (drosophila)
Aliases: AI225900; ASH1; Mash1
Human Ortholog Gene ID: [429]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding;

DNA-Binding: sequence-specific

DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Helix-Loop-Helix Family
Homolog Cluster(s): 11921


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[8662987] DNA Binding; Transactivation; TF PPI Other; Mouse Strong Co-expression of Mash1 and Mef2 in Mouse teratocarcinoma cell line P19 cells by immunoflouresence and Western blot analysis.

Transient transfections assays with MEF2A and MASH1 and CAT reporter gene (containing both a MEF2 and a MASH1 (E-box) regulatory binding sites) demonstrate transactivation when both Mef2 and Mash2 are present. Coimmunoprecipitation assays of transfected COS cells with expression vectors encoding MASH1 and MEF2A demonstrate that Mash1 and Mef2 physically interact.

Debra Fulton
[8900141] DNA Binding; Transactivation; TF PPI Human; Mouse Strong Cotransfections of MASH1, MEF2A, MEF2D, or MEF2C into 10T1/2 fibroblasts with bHLH region of E12 fused to the DNA binding domain of GAL4 activated CAT reporter assays demonstrating that MASH1 and MEF2 factors can interact and activate transcription.

Transient transfection of 10T1/2 cells of the E box (MASH1)-dependent reporter and MEF2-dependent reporter along with MASH1 and MEF2 demonstrate that MEF2 and MASH1(E1 box) can transactivate each other's binding site.

Debra Fulton
Additional Reviewer Comments:
Reviewer: Debra Fulton

Ascl1 is a transcription factor. It interacts with Mef2 (by coimmunoprecipitation and Gal3/Cat activation assays) to transactivate reporter genes.

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Facts about Ascl1 17172RDF feed
Gene description achaete-scute complex homolog 1 (drosophila)  +
Gene id 17,172  +
Gene judgment TF Gene  +
Gene symbol ascl1  +, AI225900  +, ASH1  +, and Mash1  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +, and DNA-Binding: sequence-specific  +
Homolog cluster 11,921  +
Protein family 3 Zipper-Type Group/22 Helix-Loop-Helix Family  +
Protein group 3 Zipper-Type Group   +
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