Bach2 12014

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Gene ID: 12014
Gene symbol: bach2
Gene Description: btb and cnc homology 2
Aliases: MGC117590
Human Ortholog Gene ID: [60468]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Leucine Zipper Family
Homolog Cluster(s): 107503

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[8887638] DNA Binding; Transactivation Mouse Strong Bach2 demonstrates binding as a Bach2-MafK heteromdimer to the chicken beta-globin NF-E2 response elemement (5'-TCGACCCGAAAGGAGCTGACTCATGCTAGCCC-3') in gel shift assays.

Binding site selection studies performed using a bacterially synthesized MBP-Bach2 fusion protein identified a Bach2 consensus binding site (cgTGAGTCAtc).

Transient transfection of quail QT6 fibroblasts and chicken erythroid HD3 cells show that Bach2 represses transcription mediated by a reporter plasmid containing NF-E2 sites from the chicken beta-globin 3' enhancer driving luciferase expression.

Rob Sladek
[10949928] DNA Binding Human Strong Sequence-specific DNA binding to the IgH HS3 (5'-GCTGAGGGCAGCTGAGTCATCCTGAGCCT-3') was demonstrated in BACH2-transfected RAJI cells by antibody supershift in a gel-shift assay. Rob Sladek
Additional Reviewer Comments:
Reviewer: Rob Sladek

Bach2 demonstrates binding as a Bach2-MafK heteromdimer to the chicken beta-globin NF-E2 response elemement (5'-TCGACCCGAAAGGAGCTGACTCATGCTAGCCC-3') in gel shift assays. Binding site selection studies performed using a bacterially synthesized MBP-Bach2 fusion protein identified a Bach2 consensus binding site (cgTGAGTCAtc). Transient transfection of quail QT6 fibroblasts and chicken erythroid HD3 cells show that Bach2 represses transcription mediated by a reporter plasmid containing NF-E2 sites from the chicken beta-globin 3' enhancer driving luciferase expression.


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Facts about Bach2 12014RDF feed
Gene description btb and cnc homology 2  +
Gene id 12,014  +
Gene judgment TF Gene  +
Gene symbol bach2  +, and MGC117590  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 107,503  +
Protein family 3 Zipper-Type Group/21 Leucine Zipper Family  +
Protein group 3 Zipper-Type Group   +
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