Bcor 71458

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Gene ID: 71458
Gene symbol: bcor
Gene Description: bcl6 interacting corepressor
Aliases: 5830466J11Rik; 8430401K06Rik; BcorR; D930024N20Rik; mKIAA1575
Human Ortholog Gene ID: [54880]
Reviewer Judgement: TF Gene Candidate
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 12051


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[10898795] Co-activation; TF PPI Human Medium BCoR lacks a DNA-binding domain (DBD). To test if BCoR functions as a corepressor of BCL-6, the authors fused the GAL4 DBD to BCoR and BCoR-S to determine what transcriptional effects these fusion proteins have on a GAL4-responsive reporter gene. The experiment was performed by cotransfecting HeLa cells with an SV40 promoter- and enhancer-driven, GAL4-responsive luciferase reporter (SV40 Enh G5), along with constructs for DBD alone or fusions with BCoR or BCoR-S. BCoR harbors a dose-dependent transcriptional repression activity that is not seen in BCoR-S or the GAL4 DBD alone. The DBD BCoR fusion has no effect on the reporter without the GAL4 binding sites. Thus BCoR is a corepressor that can repress transcription when physically tethered to a promoter.

The authors show that BCoR can potentiate BCL-6 repression, that it colocalizes with BCL-6 to the nucleus, that it interacts in vivo with specific class I and II histone deacetylases, and that it interacts selectively with the POZ domain of BCL-6 but not with eight other POZ proteins tested.

Shannan HoSui
[10898795] TF PPI Human Strong Yeast 2-hybrid assay showed that BCoR interacts with BCL-6, and coimmunoprecipitation assay showed that BCL-6 and BCoR interact directly in vitro and in vivo. Immunoprecipitation with deletions of BCL-6 indicate that POZ domain is responsible for interaction with BCoR. Immunofluorescence on HeLa cells transfected with BCoR and BCL-6 shows that BCoR and BCL-6 are localized together to nucleus in punctuate structures. Coimmunoprecipitation of BCoR with BCL-6 and other POZ containing proteins showed that BCoR specifically interacts with BCL-6. GAL4-BCoR fusion protein results in dose-dependent transcriptional repression activity on GAL4-responsive luciferase reporter. Cotransfection study with luciferase reporter containing BCL-6 binding sites with BCoR and BCL-6 shows increased repression compared to BCL-6 only. BCoR does not potentiate BAZF repression, where BAZF is a POZ containing repressor that does not interact with BCoR. In vivo coimmunoprecipitation study shows that BCoR coprecipitates with HDAC1,HDAC3, and HDAC-B/5. Andrew Kwon
Additional Reviewer Comments:
Reviewer: Shannan HoSui

BCOR interacts with the BCL-6 repressor protein, as shown by yeast two-hybrid analysis and chromatin immunoprecipitation. BCOR lacks a DNA-binding domain (DBD), but when tethered to a DBD is able to repress transcription of a reporter gene. It also interacts with histone deacetylases that interact with other repressor proteins.

Reviewer: Andrew Kwon

BCoR is likely a corepressor that binds to BCL-6, a known sequence-specific repressor, to increase BCL-6 repression. Immunoprecipitation studies indicate that BCL-6 and BCoR interact with each other, and cotransfection studies show that their co-presence increases the repressive activity. However, since the paper lacks an experiment to rule out sequelching of BCL-6 by BCoR, this is classified as a candidate only for now.

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Facts about Bcor 71458RDF feed
Gene description bcl6 interacting corepressor  +
Gene id 71,458  +
Gene judgment TF Gene Candidate  +
Gene symbol bcor  +, 5830466J11Rik  +, 8430401K06Rik  +, BcorR  +, D930024N20Rik  +, and mKIAA1575  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 12,051  +
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