Bhlhb2 20893

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Gene ID: 20893
Gene symbol: bhlhb2
Gene Description: basic helix-loop-helix domain containing, class b2
Aliases: C130042M06Rik; Clast5; CR8; Dec1; Sharp2; Stra13; Stra14
Human Ortholog Gene ID: [8553]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding;

DNA-Binding: sequence-specific

DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Helix-Loop-Helix Family
Homolog Cluster(s): 15206


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[12297495] DNA Binding Mouse Strong The paper identifies Stra13 as a transcriptional repressor protein that functions to regulate E-box elements through cross-interference with E-box-binding transcriptional activators that bind such sites and through direct transcriptional repression from the class B E-box and related sites. The Stra13 binding site, CACGTG, which is a class B E-box element, was identified via a PCR-based binding site selection protocol and EMSA. Competitive repression by Stra13 through class B (CACGTG) E-box elements was demonstrated in COS-7 cells or HC11 cells using luciferase reporter assays. Shannan HoSui
[12397359] DNA Binding; TF PPI Mouse Strong Both Dec1 and Dec2 suppressed Clock/Bmal1-induced activation of Per1 promoter in a dose-dependent manner as reported by a luciferase assay. Gel retardation assays show that both mouse Dec1 and mouse Dec2 bound to mouse Per1 proximal E-box elements. The binding was specific to an E-box, because excess dose of unlabelled E-box but not mutant E-box inhibited the binding. Yeast two-hybrid assay revealed that both Dec1 and Dec2 bound stereospecifically to Bmal1. Dec1 and Dec2 lacking the C-terminal regions were able to bind to Bmal1 as did the full-length Decs, but those lacking the amino terminal failed to bind, suggesting the bHLH is the responsible site. Shannan HoSui
Additional Reviewer Comments:
Reviewer: Shannan HoSui

Dec1 is a basic helix-loop-helix transcription factor involved in regulating the circadian rhythms in mammals. Dec1 and Dec2 repress Clock/Bmal1-induced transactivation of the mouse Per1 promoter through direct protein-protein interactions with Bmal1 and/or competition from E-box elements. The Dec1 binding site, CACGTG, is a class B E-box element, and was identified via a PCR-based binding site selection protocol and EMSA

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Facts about Bhlhb2 20893RDF feed
Gene description basic helix-loop-helix domain containing, class b2  +
Gene id 20,893  +
Gene judgment TF Gene  +
Gene symbol bhlhb2  +, C130042M06Rik  +, Clast5  +, CR8  +, Dec1  +, Sharp2  +, Stra13  +, and Stra14  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +, and DNA-Binding: sequence-specific  +
Homolog cluster 15,206  +
Protein family 3 Zipper-Type Group/22 Helix-Loop-Helix Family  +
Protein group 3 Zipper-Type Group   +
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