Brd4 57261

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Gene ID: 57261
Gene symbol: brd4
Gene Description: bromodomain containing 4
Aliases: Brd5; HUNK1; MCAP; WI-11513
Human Ortholog Gene ID: [23476]
Reviewer Judgement: TF Gene Candidate
Functional Classification:
Homolog Cluster(s): 14534

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[16109376] Co-activation Mouse Strong Brd4 is a bromodomain-containing protein that is recruited to mitotic chromatin by papillomavirus E2 proteins.

Dual epitope-tagged Brd4 (HA-Brd4-FLAG) was stably expressed in HeLa cells and nuclear extracts were used in a tandem affinity purification study to identify Brd4-associated proteins. The purified Brd4 complex contained Cdk9, Cyclin T1 as well as several members of the TRAP-mediator complex.

Brd4 aguments PolII CTD phosphorylation and transcription from reporter plasmids controlled by the HIV LTR following transient transfection in HeLa cells. Brd4 also aguments transcription mediated by reporters contianing the c-jun or c-myc gene promoter in NIH3T3 cells.

Chromatin immunoprecipitation studies show that Brd4 enhances pTEF-b recruitment to the HIV-LTR.

Rob Sladek
Additional Reviewer Comments:
Reviewer: Rob Sladek

Brd4 is a bromodomain-containing protein that is recruited to mitotic chromatin by papillomavirus E2 proteins. Dual epitope-tagged Brd4 (HA-Brd4-FLAG) was stably expressed in HeLa cells and nuclear extracts were used in a tandem affinity purification study to identify Brd4-associated proteins. The purified Brd4 complex contained Cdk9, Cyclin T1 as well as several members of the TRAP-mediator complex. Brd4 aguments PolII CTD phosphorylation and transcription from reporter plasmids controlled by the HIV LTR following transient transfection in HeLa cells. Brd4 also aguments transcription mediated by reporters contianing the c-jun or c-myc gene promoter in NIH3T3 cells. Chromatin immunoprecipitation studies show that Brd4 enhances pTEF-b recruitment to the HIV-LTR.


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Facts about Brd4 57261RDF feed
Gene description bromodomain containing 4  +
Gene id 57,261  +
Gene judgment TF Gene Candidate  +
Gene symbol brd4  +, Brd5  +, HUNK1  +, MCAP  +, and WI-11513  +
Homolog cluster 14,534  +
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