Cdk8 264064

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Gene ID: 264064
Gene symbol: cdk8
Gene Description: cyclin-dependent kinase 8
Aliases: MGC37111
Human Ortholog Gene ID: [1024]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 107951

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[14749387] Transactivation; TF PPI Other Medium Yeast homolog Srb10 engineered to allow inhibition in vivo, in vitro. Replacement of one a.a. residue enlarges ATP binding pocket allowing inhibition by NA-PP1, effect shown by Western. Western blot shows Srb10 present in PIC. In vitro using PIC isolated from immobilised HIS4 promoter, it is shown inhibiting Srb10 alone has negligible effect on transcription, but when inhibited in conjunction with Kin28, transcription is reduced further than when Kin28 is inhibited alone, therefore indicating that it is capable of partially enhancing transcription without Kin28. ChIP used to measure RNA Pol II cross-linking to RNA, showing significant reduction in cross-linking when Srb10 and Kin28 inhibited. Inhibition of Kin28+Srb10 shows reduction in PIC dissociation rate, Srb10 inhibited alone has negligible effect. Analysis of incorporation of 32P into PolII shows incorporation rate follows same trend as dissociation rate. TAF2 and Bdf1 identified as substrates for Srb10, by identifying weight of the substrates using immobilised template assay then confirming using PAGE and FLAG-tagging, and also Western blotting. Warren Cheung
[15546612] TF PPI Human Medium Notch signalling activates Hes1 gene, shown in vivo C33A cells by increased Hes1 RNA and levels of Hes1 proteins at various timepoints. ChIP shows CDK8 recruited to Hes1 promoter in the same timeframe that Hes1 gene is activated. It is shown to bind to MAM (Western blot). Has an inhibitory effect on luciferase-Hes1 activity. Warren Cheung
Additional Reviewer Comments:
Reviewer: Warren Cheung

Shown to associate with MAM and affect transactivation of Hes1 promoter. Yeast homolog shown to be involved with phosphorylation of RNA Pol II subunits.


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Facts about Cdk8 264064RDF feed
Gene description cyclin-dependent kinase 8  +
Gene id 264,064  +
Gene judgment TF Gene  +
Gene symbol cdk8  +, and MGC37111  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 107,951  +
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