Cic 71722

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Gene ID: 71722
Gene symbol: cic
Gene Description: capicua homolog (drosophila)
Aliases: 1200010B10Rik; mKIAA0306
Human Ortholog Gene ID: [23152]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Other Alpha-Helix Group
DBD Protein Group/Family Classification: Other Alpha-Helix Group / High Mobility Group (Box) Family
Homolog Cluster(s): 15289

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[16717057] DNA Binding; Transactivation Human Medium Human Cic weakly repressed luciferase reporter expression relative to reporter vector alone in a cotransfection assay in which an expression vector including Cic fused to the GAL4 DNA-binding domain was transfected into HeLa cells along with a reporter vector including 5 tandem copies of the GAL4 DNA-binding sequence fused to luciferase.

The HMG-box domain of Cic bound to a consensus octameric sequence within the minimal promoter of the human ERM gene (-350 to -311) in an in-vitro EMSA assay in which in-vitro translated GST-Cic (HMG-box domain) was incubated with 32P-labeled probe corresponding to the human ERM minimal promoter and then analyzed by SDS-PAGE. The binding protein was positively identified as Cic through the further addition of anti-GST antibody in a Supershift assay.

Stuart Lithwick
Additional Reviewer Comments:
Reviewer: Stuart Lithwick

Human Cic is likely a transcription factor, which represses gene expression through interactions with DNA binding elements. Cic-GAL4DBD represses luciferase expression when transfected into HeLa cells with a luciferase reporter vector. Also, the HMG-box domain of Cic binds to an 8 bp sequence within the human ERM minimal promoter as detected by EMSA and Supershift assays.


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Facts about Cic 71722RDF feed
Gene description capicua homolog (drosophila)  +
Gene id 71,722  +
Gene judgment TF Gene  +
Gene symbol cic  +, 1200010B10Rik  +, and mKIAA0306  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 15,289  +
Protein family 4 Other Alpha-Helix Group/28 High Mobility Group (Box) Family  +
Protein group 4 Other Alpha-Helix Group   +
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