Creb1 12912

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Gene ID: 12912
Gene symbol: creb1
Gene Description: camp responsive element binding protein 1
Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; Creb; Creb-1
Human Ortholog Gene ID: [1385]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Leucine Zipper Family
Homolog Cluster(s): 107503

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[14593102] DNA Binding; Transactivation Mouse Strong C/EBP-Beta promoter construct-luciferase reporter assays and site-directed mutatgennsis identified the minimum proximal promoter (-117 bp) and binding elements required for maximum activity of C/EBP{beta} promoter reporter.

Supershift experiments using co-incubation of antibodies against CREB family members and nuclear extracts from 3T3-L1 preadipocytes (after exposure to the adipocyte differentiation inducers (MDI)) identify binding of CREB and ATF1 proteins to C/EBP-beta binding elements. Co-transfection of 3T3-L1 preadipocytes with a C/EBP{beta} promoter-luciferase construct and expression vectors for CREB, CREM{tau}, or ATF1 demonstrate that CREB transactivates the C/EBP-beta promoter.

Debra Fulton
[15054091] Other; DNA Binding Mouse Strong Gel-shift assays and competition experiments using nuclear proteins from N2A cells and an oligoprobe encompassing an AP-1 binding site from the murine GLUT gene promoter with the presence of anti-CREB antibody demonstrate CREB binding.

Co-transfection experiments employing a CREB expression vector and the -203-bp GLUT 3-Luciferase reporter construct were unsuccessful because of high levels of endogenous CREB present in most cell lines investigated. However, SiRNA against the CREB sequence (confirmed by Western analysis) and the -203-bp GLUT 3 promoter-luciferase reporter DNA depletes the activation of the reporter gene.

Debra Fulton
Additional Reviewer Comments:
Reviewer: Debra Fulton

Creb is a transcription factor. It transactivates a C/EBP-beta reporter gene and siRNA against the CREB sequence depletes the activation of the GLUT 3 reporter gene.


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Facts about Creb1 12912RDF feed
Gene description camp responsive element binding protein 1  +
Gene id 12,912  +
Gene judgment TF Gene  +
Gene symbol creb1  +, 2310001E10Rik  +, 3526402H21Rik  +, AV083133  +, Creb  +, and Creb-1  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 107,503  +
Protein family 3 Zipper-Type Group/21 Leucine Zipper Family  +
Protein group 3 Zipper-Type Group   +
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