Creb3l3 208677

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Gene ID: 208677
Gene symbol: creb3l3
Gene Description: camp responsive element binding protein 3-like 3
Aliases: BC010786; CREB-H; D10Bur1e
Human Ortholog Gene ID: [84699]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Leucine Zipper Family
Homolog Cluster(s): 107503


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[11353085] DNA Binding; Transactivation Human Strong In the initial screening experiment with RT�PCR, the authors observed the liver-specific expression of CREB-H using 16 human tissues. To confirm this observation, they performed northern blotting analysis with the 16 human tissues.

To determine whether CREB-H is a transcription activator, plasmids expressing GAL4�CREB-H fusion proteins were produced and co-transfected with a luciferase-reporter plasmid containing the GAL4 DNA-binding element into COS7 cells. CREB-H functioned as a transcriptional activator and its N-terminal 149 amino acids accounted for the activation ability.

Fusion proteins of GST and CREB-H lacking the putative transmembrane domain were produced in bacteria and purified by glutathione�Sepharose 4B. The Gel mobility shift assays revealed that CREB-H did not bind to the C/EBP, AP-1 and NF-B elements but specifically bound to CRE and the box-B element. Luciferase reporter assays demonstrated that like BBF-2, CREB-H activated transcription via the box-B element and that a deletion of the putative transmembrane domain increased the activation of reporter expression significantly.

Using COS7 cells, a fusion protein of GFP and full-length CREB-H was localized in reticular structures surrounding the nucleus, whereas a fusion protein of GFP and a deletion mutant lacking the putative transmembrane domain was mainly in the nucleus.

Shannan HoSui
Additional Reviewer Comments:
Reviewer: Shannan HoSui

CREB-H is a liver-enriched transcription factor that binds specifically to CRE and box-B elements. DNA binding has been demonstrated using gel mobility shift assays. CREB-H acts as a transactivator, as shown by lucerifase assay. Deletion of the putative transmembrane domain increased activation, suggesting that the transmembrane domain may be involved in modulating activity.

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Facts about Creb3l3 208677RDF feed
Gene description camp responsive element binding protein 3-like 3  +
Gene id 208,677  +
Gene judgment TF Gene  +
Gene symbol creb3l3  +, BC010786  +, CREB-H  +, and D10Bur1e  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 107,503  +
Protein family 3 Zipper-Type Group/21 Leucine Zipper Family  +
Protein group 3 Zipper-Type Group   +
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