Ddb2 107986

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Gene ID: 107986
Gene symbol: ddb2
Gene Description: damage specific dna binding protein 2
Aliases: 2610043A19Rik; MGC117576
Human Ortholog Gene ID: [1643]
Reviewer Judgement: Not a TF - Evidence that it is not a TF; TF Gene Candidate
Functional Classification: Single stranded RNA/DNA binding;

Transcription Factor Binding: tf co-factor binding

Homolog Cluster(s): 107986

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[9418871] Co-activation Human Strong Expression of DDB stimulated transcription from the E2F1 promoter (CAT reporter assay). The interaction between DDB and E2F1 can also be detected by coimmunoprecipitation experiments.

Used Gal4 fusion constructs containing E2F1 and a reporter CAT gene containing five Gal4-binding sites demonstrate that DDB associates with the activation domain of E2F1 and stimulate E2F1-activated transcription.

Debra Fulton
[12145536] Non-specific DNA Human Strong Damaged-DNA recognition activity has been identified as a complex of DDB1 (127-kD) and DDB2 (48-kD) subunits. Over-expression of DDB1 did not augment UV-damaged-DNA recognition activity in human HeLa, hamster V79, or rat PC12 cells. Restricting DDB2 expression by antisense ddb2 partially inhibited UV-damaged-DNA recognition activity in cells and overexpressing DDB2 through a recombinant ddb2 adenovirus partly restored the recognition activity of these cells.

UV-nonspecific DNA binding for DBD1 and DBD2 was demonstrated with EMSA and immunoblot analysis.

Debra Fulton
[12826282] Other Mouse Weak studies suggest ddb2 acts in the "global nucleotide excision repair (NER) pathway". Seems to be indirect links to p53 and transcriptional regulation. Jared Roach
[9418871] Transactivation Human Strong p48 subunit of DDB2 binds to and activates E2F1. Binding of DDB2 to damaged DNA prevents E2F1-mediated transcrition of cell-cycle genes. Sarav Sundararajan
[12034848] TF PPI Human Strong DDB2 binds p300. Rob Sladek
Additional Reviewer Comments:
Reviewer: Jared Roach

This protein appears to play a role as a DNA damage repair protein. I do not see evidence that it plays a role as a transcription factor. However, such evidence might exist or eventually be found. At this stage it appears to be involved with DNA but not as TF

Reviewer: Rob Sladek

DDB1 (p127) and DDB2 (p48) form subunits of the damage-specific DNA binding protein. The DDB1 subunit binds DNA nonspecifically to DNA containing UV radiation photoproducts, while the DDB2 subunit interacts with p300.


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Facts about Ddb2 107986RDF feed
Gene description damage specific dna binding protein 2  +
Gene id 107,986  +
Gene judgment Not a TF - Evidence that it is not a TF  +, and TF Gene Candidate  +
Gene symbol ddb2  +, 2610043A19Rik  +, and MGC117576  +
Gene taxonomy Single stranded RNA/DNA binding  +, and Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 107,986  +
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