Fli1 14247

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Gene ID: 14247
Gene symbol: fli1
Gene Description: friend leukemia integration 1
Aliases: EWSR2; Fli-1; SIC-1; Sic1
Human Ortholog Gene ID: [2313]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Winged Helix-Turn-Helix
DBD Protein Group/Family Classification: Winged Helix-Turn-Helix / Ets Domain Family
Homolog Cluster(s): 104156

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[12065417] DNA Binding; TF PPI Mouse Strong Fli-1 binds to a SCL stem cell enhancer (EMSA, chIP).

It forms an enhanceosome with GATA-2 and E74-like factor 1 on this enhancer as shown by the EMSA and GST pull-down experiments.

Elodie Portales-Casamar
[14570912] DNA Binding; Transactivation Mouse Strong DNA binding specificity of FLI-1 shown using EMSA experiments.

FLI-1transactivates the ETS-responsive region-1 of the human T-cell leukemia virus type I-long terminal repeat in a gene reporter assay.

Elodie Portales-Casamar
[1445800] Transactivation Mouse Strong Human ERGB and its Mouse homolog Fli1 are characterized in this paper. These genes contain an ETS motif. Similar to the other members of the ets family of transcription factor (ets 1, ets 2), this new member is also able to trans-activate transcription of a reporter gene linked to the ETS-binding sequences derived from either the GATA-1 promoter or an optimal Ets-binding site. Gwenael Breard
[1522903] DNA Binding Human Medium The authors demonstrated that the translocation t(11;22)(q24;q12) substitutes a putative RNA-binding domain of the Ewing sarcoma gene on chromosome 22 for the DNA-binding domain of the FLI1 gene on chromosome 11. They found that the breakpoints on chromosome 22 were clustered within a 7-kb region, whereas the breakpoints on chromosome 11 were scattered over a 40-kb region, termed EWSR2.

Another study confirmed later that the 11;22 translocation of Ewing sarcoma produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation.

Gwenael Breard
[8449942] DNA Binding Mouse Strong Analysis of the human and mouse fli-1 proteins showed that fli-1 binds to specific DNA sequences highly related to m-ets-2 binding sites. Methylation protection experiments showed that fli-1 and m-ets-2 contacted the same nucleotides in two different binding sites. Gwenael Breard
[8502483] DNA Binding Human Strong In this study, the authors show that the Fli-1 protein, like other Ets proteins, has DNA-binding activity and can act as a sequence-specific transcriptional activator. Furthermore, they have identified an octanucleotide core sequence that is required in vitro for optimal binding of Fli-1 to the Drosophila E74 target and the promoter sequence of the human GPIIB gene Gwenael Breard
Additional Reviewer Comments:
Reviewer: Gwenael Breard

Fli1 belongs to the ETS family of transcription factor. It has been shown in human that the translocation t(11;22)(q24;q12) substitutes a putative RNA-binding domain of the Ewing sarcoma gene on chromosome 22 for the DNA-binding domain of the FLI1 gene on chromosome 11.This translocation of Ewing sarcoma produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation.

Reviewer: Elodie Portales-Casamar

strong evidence of specific binding and transactivation of different promoters.


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Facts about Fli1 14247RDF feed
Gene description friend leukemia integration 1  +
Gene id 14,247  +
Gene judgment TF Gene  +
Gene symbol fli1  +, EWSR2  +, Fli-1  +, SIC-1  +, and Sic1  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 104,156  +
Protein family 1.2 Winged Helix-Turn-Helix/16 Ets Domain Family  +
Protein group 1.2 Winged Helix-Turn-Helix   +
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