Fosb 14282

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Gene ID: 14282
Gene symbol: fosb
Gene Description: fbj osteosarcoma oncogene b
Aliases:
Human Ortholog Gene ID: [2354]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 107503

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[2498083] TF PPI Human Medium EMSA binding studies and quantitation analysis between in vitro synthesized c-fos, fos B, c-jun and jun B proteins and a 32P-labelled AP-1 binding consensus sequence or TRE (TPA response element) oligodeoxynucleotide demonstrate that combinations of c-fos or fos B proteins with c-jun or jun B proteins interact and are more efficient in binding DNA.

Immunoprecipitation studies either on single c-jun, c-fos or fos B proteins or on combinations of them with antic-jun, anti-c-fos and anti-fos B antisera demonstrate that combinations of c-jun and c-fos and c-jun and fos B proteins co-immunoprecipitate together suggesting that their interaction occurs in the absence of the target binding sequence.

Debra Fulton
[14515349] TF PPI Mouse Strong Densitometric analysis of EMSA gels (with radiolabled AP1 probe) determine that the addition of both SPD and SPN significantly increase AP1 DNA binding in a concentration-dependent manner.

Supershift antibody EMSAs using nuclear extracts (from discrete murine brain structures) and incubated with radiolabeled AP1 probe followed by reaction with one of antibodies against c-Jun, Jun-B, Jun-D, c-Fos, Fos-B, Fra-1, and Fra-2 proteins demonstrate that ti-Fos-B antibody was also effective in inducing supershift of AP1 DNA binding in the presence of SPD and SPN (Western blotting). Immunoprecipitation against Fos-B protein followed by densitometric analysis of EMSA gels (with radiolabled AP1 probe and addition of SPD and SPN) determine that neither SPD nor SPN (without Fos-B) can significantly increased AP1 DNA binding in nuclear extracts prepared from neocortex and hippocampus.

Debra Fulton
[16699069] DNA Binding; Transactivation; TF PPI Rat; Mouse Strong Ischemia-reperfusion induces cardiac MMP-2 gene transcription using a

rat MMP-2 promoter extending to �1686 bp relative to the translational start site was used to drive expression of an E. coli -galactosidase cassette in ( CD-1 background strain) transgenic mice. Quantitative real-time PCR assessment of intrinsic MMP-2 transcription and Western blot quantitation of MMP-2 protein expression after ischemia-reperfusion injury demonstrates that after 30 min of ischemia and 90 min of reperfusion, there is a 2.8-fold increase in MMP-2 transcript abundance, which was blocked by perfusion with the free radical scavenger MPG. EMSA of ventricular nuclear extracts demonstrates specific nuclear protein-DNA interactions with the 1420- to 1362-bp oligonucleotide containing the activator proteins-1 (AP-1) site. EMSA of ventricular nuclear proteins isolated from control hearts using antibodies to each Fos and Jun family member demonstrate AP-1 site nuclear binding proteins as FosB and JunB. Chromatin immunoprecipitation (ChIP) assay demonstates JunB/FosB binding to the intrinsic (murine genomic) MMP-2 promoter.

Debra Fulton
Additional Reviewer Comments:
Reviewer: Debra Fulton

Fos-B can interact with cjun or jun B/Fox-B (as demonstrated by EMSA and quantitation binding studies). Chromatin immunoprecipitation (ChIP) assay demonstates JunB/FosB binding to the intrinsic (murine genomic) MMP-2 promoter. EMSA of ventricular nuclear extracts demonstrates specific nuclear protein-DNA interactions containing the activator proteins-1 (AP-1) site in the MMP-2 promoter. EMSA of ventricular nuclear proteins isolated from control hearts using antibodies to each Fos and Jun family member demonstrate AP-1 site nuclear binding proteins as FosB and JunB.


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Facts about Fosb 14282RDF feed
Gene description fbj osteosarcoma oncogene b  +
Gene id 14,282  +
Gene judgment TF Gene  +
Gene symbol fosb  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 107,503  +
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