Gcm2 107889

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Gene ID: 107889
Gene symbol: gcm2
Gene Description: glial cells missing homolog 2 (drosophila)
Aliases: Gcm-rs1; Gcm1; Gcm1-rs2; MGC130289; MGC130290
Human Ortholog Gene ID: [9247]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zinc-coordinating Group
DBD Protein Group/Family Classification: Zinc-coordinating Group / Glial Cells Missing (GCM) Domain Family
Homolog Cluster(s): 107889


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[10671510] DNA Binding; Transactivation Human; Other; Mouse Strong Whole cell extracts from COS cells transfected with an expression plasmid for the DNA-binding domain of mouse GCMb (Gcm2) and 5'-ATGCGGGT-3' consensus sequence oligonucleotides demonstrates mGCMb DNA-binding with high affinity (by EMSA).

Transfection of a luciferase reporter plasmid carrying six tandemly arranged GCM binding sites into U138 glioblastoma cells, COS cells, 293 cells, and Drosophila S2 cells demonstrates that GCMb stimulates the reporter gene but less so than GCMa. Cotransfection in 293 cells with chimera-reporters between both proteins GCMb and GCMa (Chimera C1 containing the DNA-binding domain of mGCMa in the context of mGCMb sequences and Chimera C2 containing the DNA-binding domain of mGCMb with all other sequences being derived from mGCMa) and expression plasmids for mGCMa and mGCMb demonstrate that transactivation differences are due to the carboxyl-terminal to the DNA-binding domain. Gal4 reporter fusions between various regions of mGCMb and the transcriptionally inactive POU domain of Tst-1/Oct6/SCIP in transfected 293 cells demonstrates that transactivation domains are present within mGCMb.

mGCMb was expressed from its CMV-driven effector plasmid and was detected in its epitope-tagged version in COS cell nuclear extracts by Western blot analysis

Debra Fulton
Additional Reviewer Comments:
Reviewer: Debra Fulton

Gcm2 is a transcription factor. It binds a consensus DNA sequence (by EMSA) and differentially transactivates a GCM binding element-reporter in U138 glioblastoma cells, COS cells, 293 cells, and Drosophila S2 cells.

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Facts about Gcm2 107889RDF feed
Gene description glial cells missing homolog 2 (drosophila)  +
Gene id 107,889  +
Gene judgment TF Gene  +
Gene symbol gcm2  +, Gcm-rs1  +, Gcm1  +, Gcm1-rs2  +, MGC130289  +, and MGC130290  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 107,889  +
Protein family 2.1 Zinc-coordinating Group/105 Glial Cells Missing (GCM) Domain Family  +
Protein group 2.1 Zinc-coordinating Group   +
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