Batf 53314

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Gene ID: 53314
Gene symbol: batf
Gene Description: basic leucine zipper transcription factor, atf-like
Aliases: B-ATF; SFA-2
Human Ortholog Gene ID: [10538]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 107503

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[10777209] Transactivation; TF PPI Human; Mouse Strong Transient transfection of various portions of the B-ATF protein (expressed as fusions with the DNA binding domain of the yeast GAL4 protein) in C3H10T1/2 cells demonstrate that the B-ATF fusion protein does not activate transcription suggesting that B-ATF in AP-1 complexes is unlikely to enhance transcription.

B-ATF interacts with JunB through the B-ATF leucine zipper motif by two-hybrid, protein-protein interaction assay in C3H10T1/2 cells. Jun/B-ATF heterodimers demonstrate a preference for binding the AP-1 consensus DNA by EMSA (competition and mutational analysis) assays. Cotransfectrin of a luciferase reporter gene controlled by three tandem AP-1 consensus sites, in mouse F9 teratocarcinoma cells (F9 cells display a low level of endogenous AP-1 activity) with c-FOS and with or without B-ATF demonsrates that B-ATF represses the c-Fos activation by 50%. A B-ATF VP16 TAD fusion protein activates AP-1 reporter gene activity in C3H10T1/2 cells demonstrating that B-ATF functions as a non-activating competitor of c-Fos in the AP-1 dimer. A C3H10T1/2 cell line stably expressing moderate levels of a hemaglutinin-antigen (HA)-tagged human B-ATF protein indicates B-ATF as a negative regulator of AP-1-mediated activities.

Debra Fulton
[11466704] Transactivation Mouse Medium BATF negatively regulates the transactivation of an AP-1 reporter gene in vivo (by transgenic mouse lines constitutively expressing BATF in thymocytes and an HA-BATF transgene controlled by a proximal promoter). Debra Fulton
Additional Reviewer Comments:
Reviewer: Debra Fulton

B-ATF is a transcription factor. B-ATF interacts with JunB through the B-ATF leucine zipper motif by two-hybrid-protein-protein interaction assays. Jun/B-ATF heterodimers demonstrate a preference for binding the AP-1 consensus DNA by EMSA (competition and mutational analysis) assays. B-ATF functions as a non-activating competitor of c-Fos in the AP-1 dimer by VP16 TAD fusion assays. B-ATF is a negative regulator of AP-1 mediated activities (as demonstrated by stabe expression of HA-taggged B-ATF in C3H10T1/2 mouse fibroblasts and a mouse transgenic line).


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Facts about Batf 53314RDF feed
Gene description basic leucine zipper transcription factor, atf-like  +
Gene id 53,314  +
Gene judgment TF Gene  +
Gene symbol batf  +, B-ATF  +, and SFA-2  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 107,503  +
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