Ctcf 13018
From TFCatWiki
(Redirected from MGI:109447)
External Links
| Gene ID: | 13018 |
| Gene symbol: | ctcf |
| Gene Description: | ccctc-binding factor |
| Aliases: | AW108038 |
| Human Ortholog Gene ID: | [10664] |
| Reviewer Judgement: | TF Gene |
| Functional Classification: | DNA-Binding: sequence-specific |
| DBD Protein Group Classification: | Zinc-coordinating Group |
| DBD Protein Group/Family Classification: | Zinc-coordinating Group / BetaBetaAlpha-zinc finger Family |
| Homolog Cluster(s): | 107586 |
Evidence:
| Pubmed ID | Function | Species | Evidence Strength | Reviewer's Comments | Reviewer |
| [8246978] | DNA Binding; Transactivation | Other | Strong | CTCF is purified using sequence-specific chromatography with chicken c-myc 5'-flanking region and checked using EMSA and DNaseI footprinting. Missing-contact probing involving EMSA with mutated oligos is used to find the bases that contact CTCF. CAT reporter construct with wild type and mutated c-myc CTCF binding sites shows that the mutation that eliminates CTCF-binding decrease reporter expression. | Andrew Kwon |
| [15143173] | DNA Binding | Mouse | Strong | EMSA with wild type and mutated H19/Igf2 ICR shows that only the wild type binds to the ICR. This binding is competed by unlabeled oligos and supershifted by anti-CTCF Ab. | Andrew Kwon |
| Additional Reviewer Comments: |
Reviewer: Andrew Kwon EMSA, cotransfection, and DNaseI footprinting assay shows that CTCF binds to a specific sequence and activate transcription. CTCF is also involved in imprinting and acts as insulators. |
| DISCUSSION PAGE link for this gene |
|
VIEW gene annotation feedback in the [TFCat Annotation Feedback Reports Inquiry] |
|
REPORT gene annotation feedback on the [TFCat Annotation Feedback Reporting System] Help is available here |
| The section below defines information for semantic searching. Please see the help page for more information on this section. |
Facts about Ctcf 13018RDF feed
| Gene description | ccctc-binding factor + |
| Gene id | 13,018 + |
| Gene judgment | TF Gene + |
| Gene symbol | ctcf +, and AW108038 + |
| Gene taxonomy | DNA-Binding: sequence-specific + |
| Homolog cluster | 107,586 + |
| Protein family | 2.1 Zinc-coordinating Group/17 BetaBetaAlpha-zinc finger Family + |
| Protein group | 2.1 Zinc-coordinating Group + |
