Ascl3 56787

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Gene ID: 56787
Gene symbol: ascl3
Gene Description: achaete-scute complex homolog 3 (drosophila)
Aliases: MGC129412; MGC129413; Sgn1
Human Ortholog Gene ID: [56676]
Reviewer Judgement: TF Gene Candidate
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Helix-Loop-Helix Family
Homolog Cluster(s): 11921


Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[11784080] Co-activation; DNA Binding; TF PPI Human; Mouse Medium A mobility shift was detected in an in-vitro EMSA assay in which Ascl3 (referred to by its alias Sgn1 in this PMID) interacts with a synthetic oligonucleotide generated through Cycling Amplification and Selection of the Target (CASTing) in an in-vitro EMSA assay, in which in-vitro translated mouse Sgn1 was incubated with 32P-labeled synthetic oligonucleotide selected through Cycling Amplification and Selection of the Target (CASTing).

MyoD and Mash1 of unspecified species both activated reporter gene expression when separately transfected into C3H10T1/2 mouse fibroblasts in addition to a luciferase reporter vector (six tandem repeats of putative Ascl3-E47 binding site "oligo A" -MEF-1 sequences-SV40 promoter-luciferase). A decrease in reporter expression was observed upon the additional transfection of an expression vector encoding Ascl3.

Stuart Lithwick
Additional Reviewer Comments:
Reviewer: Stuart Lithwick

Ascl3 may be a transcription factor, which regulates gene expression. Ascl3 interacts with an oligonucleotide selected using CASTing as demonstrated by EMSA assay. However, competition assays were not included, lessening the impact of this data. In addition, Ascl3 reduces reporter gene expression when cotransfected into mouse fibroblasts with expression vectors for MyoD and Mash1 as well as a reporter vector construct. In the absence of Ascl3, the cotransfection of expression vectors for either MyoD or Mash1 with the reporter vector construct leads to increased reporter expression.

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Facts about Ascl3 56787RDF feed
Gene description achaete-scute complex homolog 3 (drosophila)  +
Gene id 56,787  +
Gene judgment TF Gene Candidate  +
Gene symbol ascl3  +, MGC129412  +, MGC129413  +, and Sgn1  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 11,921  +
Protein family 3 Zipper-Type Group/22 Helix-Loop-Helix Family  +
Protein group 3 Zipper-Type Group   +
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