Neurod1 18012

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Gene ID: 18012
Gene symbol: neurod1
Gene Description: neurogenic differentiation 1
Aliases: BETA2; BHF-1; Neurod
Human Ortholog Gene ID: [4760]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Helix-Loop-Helix Family
Homolog Cluster(s): 11921

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[9111329] DNA Binding; Transactivation Other Strong Neurod1 interacts with an E box in the STF1 pancreatic islet-specific enhancer in gel shift assays performed using nuclear extracts from HIT-T15 cells. Participation of Neurod1and E47 in the retarded complex was onfirmed by antibody supershifting studies. Cotransfected Neurod1 and E47 activate a reporter controlled by a minimal STF1 islet enhancer (bases -6200 to -5700) following transient transfection in HeLa cells. Rob Sladek
[9343431] DNA Binding; Transactivation Other Strong Neurod1 acting as a heterodimer with other bHLH factors, induces transcription from a reporter controlled by the proopiomelanocortin (POMC) gene promoter (bases -480 to +63). Gel shift assays, performed using nuclear extracts from transfected L cells and untransfected At-T20 cells, demonstrated that Neurod1 interacted with probes derived from POMC E-box sequences. Participation of Neurod1 in the retarded complex, for both At-T20 and transfected L cells, was confirmed by antibody supershift. Rob Sladek
Additional Reviewer Comments:
Reviewer: Rob Sladek

Neurod1 interacts with an E box in the STF1 pancreatic islet-specific enhancer in gel shift assays performed using nuclear extracts from HIT-T15 cells. Participation of Neurod1and E47 in the retarded complex was onfirmed by antibody supershifting studies. Cotransfected Neurod1 and E47 activate a reporter controlled by a minimal STF1 islet enhancer (bases -6200 to -5700) following transient transfection in HeLa cells. Neurod1 acting as a heterodimer with other bHLH factors, induces transcription from a reporter controlled by the proopiomelanocortin (POMC) gene promoter (bases -480 to +63). Gel shift assays, performed using nuclear extracts from transfected L cells and untransfected At-T20 cells, demonstrated that Neurod1 interacted with probes derived from POMC E-box sequences. Participation of Neurod1 in the retarded complex, for both At-T20 and transfected L cells, was confirmed by antibody supershift.


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Facts about Neurod1 18012RDF feed
Gene description neurogenic differentiation 1  +
Gene id 18,012  +
Gene judgment TF Gene  +
Gene symbol neurod1  +, BETA2  +, BHF-1  +, and Neurod  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 11,921  +
Protein family 3 Zipper-Type Group/22 Helix-Loop-Helix Family  +
Protein group 3 Zipper-Type Group   +
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