Nfe2l2 18024

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Gene ID: 18024
Gene symbol: nfe2l2
Gene Description: nuclear factor, erythroid derived 2, like 2
Aliases: AI194320; Nrf2
Human Ortholog Gene ID: [4780]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zipper-Type Group
DBD Protein Group/Family Classification: Zipper-Type Group / Leucine Zipper Family
Homolog Cluster(s): 107503

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[15728556] DNA Binding Human Strong NFE2L2 bound an antioxidant response element (ARE) oligonucleotide in EMSA on human K-1034 cells. Band shift was also induced by small Maf antibody (recognizes MafK, MafG, MafF) and by c-JUN antibody (recognizes c-Jun, JunB and JunD) but not by controls. Stefanie Butland
[16545679] DNA Binding; Transactivation Human Strong EMSA band shift and supershift with nuclear extracts from Hep-G2 and HeLa cells, and ChIP assays with Hep-G2 cells demonstrate that NRF2 binds an oligonucleotide corresponding to an antioxidant response element (ARE) in human NQO2 (NRH:quinone oxidoreductase).

Reporter gene assay with NFE2L2 coexpressed with NQO2 promoter-luciferase construct in Hep-G2 cells showed transactivation. Dominant-negative NFE2L2 mutant or NFE2L2 siRNA both negatively regulated NQO2 gene promoter activity.

Stefanie Butland
Additional Reviewer Comments:
Reviewer: Stefanie Butland

NFE2L2 is a transcription factor. Gel shift assays show that it specifically recognizes antioxidant response element (ARE) DNA sequences. Transient transfection assays show that NFE2L2 activates transcription of the NRH:quinone oxidoreductase gene.


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Facts about Nfe2l2 18024RDF feed
Gene description nuclear factor, erythroid derived 2, like 2  +
Gene id 18,024  +
Gene judgment TF Gene  +
Gene symbol nfe2l2  +, AI194320  +, and Nrf2  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 107,503  +
Protein family 3 Zipper-Type Group/21 Leucine Zipper Family  +
Protein group 3 Zipper-Type Group   +
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