Rora 19883

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Gene ID: 19883
Gene symbol: rora
Gene Description: rar-related orphan receptor alpha
Aliases: 9530021D13Rik; nmf267; Nr1f1; ROR1; ROR2; ROR3; sg; staggerer
Human Ortholog Gene ID: [6095]
Reviewer Judgement: TF Gene
Functional Classification: DNA-Binding: sequence-specific
DBD Protein Group Classification: Zinc-coordinating Group
DBD Protein Group/Family Classification: Zinc-coordinating Group / Hormone-nuclear Receptor Family
Homolog Cluster(s): 110784

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[15821743] DNA Binding Mouse Strong Paper: The orphan nuclear receptor ROR regulates circadian transcription of the mammalian core-clock Bmal

Overexpression of ROR1 or ROR4 protein strongly promoted Bmal1 transcription (luciferase assays) DNA-binding shown: isolated complexes between the Bmal1 promoter and ROR protein through pull-down experiments with the promoter fragment.

Debra Fulton
[7926749] DNA Binding Unknown Strong "Three isoforms of a novel member of the steroid hormone nuclear receptor superfamily related to the retinoic acid receptors have been identified. The three isoforms, referred to as ROR alpha 1, ROR alpha 2, and ROR alpha 3, share common DNA- and putative ligand-binding domains but are characterized by distinct amino-terminal domains generated by alternative RNA processing. An exon encoding a functionally important subregion of the amino-terminal domain of the ROR alpha 2 isoform resides on the opposite strand of a cytochrome c-processed pseudogene. Binding site selection using in vitro-synthesized proteins reveals that the ROR alpha 1 and ROR alpha 2 isoforms bind DNA as monomers to hormone response elements composed of a 6-bp AT-rich sequence preceding a half-site core motif PuGGTCA (RORE). However, ROR alpha 1 and ROR alpha 2 display different binding specificities: ROR alpha 1 binds to and constitutively activates transcription from a large subset of ROREs, whereas ROR alpha 2 recognizes ROREs with strict specificity and displays weaker transcriptional activity. The differential DNA-binding activity of each isoform maps to their respective amino-terminal domains. Whereas truncation of the amino-terminal domain diminishes the ability of ROR alpha 1 to bind DNA, a similar deletion relaxes ROR alpha 2-binding specificity to that displayed by ROR alpha 1. Remarkably, transfer of the entire amino-terminal region of ROR alpha 1 or amino-terminal deletion of ROR alpha 2 confers RORE-binding specificities to heterologous receptors. These results demonstrate that the amino-terminal domain and the zinc finger region work in concert to confer high affinity and specific DNA-binding properties to the ROR isoforms and suggest a novel strategy to control DNA-binding activity of nuclear receptors." Jared Roach
[7926749] DNA Binding Human Strong Describes a DNA binding site selection screen and determines domains associated with DNA binding Sarav Sundararajan
[15941850] Transactivation Human Strong Identified RORa as a positive regulator of fibrinogen-beta gene expression both in human hepatoma cells and in mouse liver by EMSA and luciferase reporter assays. Sarav Sundararajan
[7926749] DNA Binding Human Strong RORalpha1 and RORalpha2 display different DNA binding specificities based on results of binding site studies. Rob Sladek
[12467577] Transactivation Human Strong Transcriptional activity of RORαlpha is modulated by changes in intracellular cholesterol level in transient transfection studies. Rob Sladek
Additional Reviewer Comments:
Reviewer: Jared Roach

Nucelar hormone receptor.


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Facts about Rora 19883RDF feed
Gene description rar-related orphan receptor alpha  +
Gene id 19,883  +
Gene judgment TF Gene  +
Gene symbol rora  +, 9530021D13Rik  +, nmf267  +, Nr1f1  +, ROR1  +, ROR2  +, ROR3  +, sg  +, and staggerer  +
Gene taxonomy DNA-Binding: sequence-specific  +
Homolog cluster 110,784  +
Protein family 2.1 Zinc-coordinating Group/18 Hormone-nuclear Receptor Family  +
Protein group 2.1 Zinc-coordinating Group   +
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