Blzf1 66352

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Gene ID: 66352
Gene symbol: blzf1
Gene Description: basic leucine zipper nuclear factor 1
Aliases: 1700030G05Rik; Blzf1l; Golgin-45; Jem-1
Human Ortholog Gene ID: [8548]
Reviewer Judgement: TF Gene Candidate
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 66352

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[9129147] Other Human Weak nuclear localization

cloning

Wyeth Wasserman
[10602419] Co-activation Human Weak DNA mobility shift assays showed that the in vitro translated Jem protein interacts neither with the DNA binding site of AP-1, nor directly with in vitro co-translated c-Fos or/and c-Jun proteins bound to this specific sequence. Interestingly, Jem-1-1 increased substantially the transcriptional activity of c-Jun (three-fold) and more strongly that of ectopically co-expressed c-Fos and c-Jun (five- to six-fold), as measured by a CAT reporter gene driven by a heterologous promoter containing the AP-1 binding site of the human collagenase gene. These synergistic effects were strongly Jem-1 dose-dependent. However, Jem-1 alone showed no activity on the collagenase promoter. A deletion of the leucine repeat of Jem-1 (Arg179 --> Glu206) did not diminish the enhancer capacity of Jem-1 on AP-1 activity. In contrast, the enhanced AP-1 activity was abrogated when Jem-1 was deleted of its C-terminus (Thr331 --> Leu400). We conclude that the 45 kDa nuclear product of the JEM-1 gene has features of a novel transcription cofactor, which is enhancing AP-1 activity without directly interacting with c-Jun or c-Fos proteins. Wyeth Wasserman
Additional Reviewer Comments:
Reviewer: Wyeth Wasserman

No evidence for sequence specific DNA binding (not ruled out); suffficient evidence tha tit interacts with other TFs and can modulate their activity


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Facts about Blzf1 66352RDF feed
Gene description basic leucine zipper nuclear factor 1  +
Gene id 66,352  +
Gene judgment TF Gene Candidate  +
Gene symbol blzf1  +, 1700030G05Rik  +, Blzf1l  +, Golgin-45  +, and Jem-1  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 66,352  +
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