Ep300 328572

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Gene ID: 328572
Gene symbol: ep300
Gene Description: e1a binding protein p300
Aliases: A430090G16; A730011L11; p300
Human Ortholog Gene ID: [2033]
Reviewer Judgement: TF Gene
Functional Classification: Transcription Factor Binding: tf co-factor binding
Homolog Cluster(s): 12914

Evidence:

Pubmed ID Function Species Evidence Strength Reviewer's Comments Reviewer
[11481322] Co-activation Human Strong p300 coactivates GATA-4 transcription on natural and artificial cardiac promoters (gene reporter assays in NIH3T3 cells using constructs carrying either 6 copies of GATA-binding sites or proximal promoters of ANF or the beta -myosin heavy chain).

GATA-4 and p300 Physically Interact (co-IP of overexpressed proteins in COS cells). The interacting Domain of p300 with GATA-4 Can Inhibit GATA-4 Transcription (gene reporter assays in NIH3T3 cells). The acetyltransferase activity of p300 is required for coactivation of GATA-4 transcription (gene reporter assays in NIH3T3 cells using an HAT-inactive p300 mutant).

Elodie Portales-Casamar
[12586840] Co-activation Human Strong p300 and CBP synergize with CITED2 to co-activate TFAP2A (gene reporter assay with a p3xAP2-Bluc reporter in Hep3B or HepG2 cells).

TFAP2A interacts physically with p300 in vivo (co-IP of overexpressed proteins in U2-OS cells). The HAT-deficient p300 mutant does not co-activate TFAP2A (gene reporter assay with a p3xAP2-Bluc reporter in Hep3B or HepG2 cells). p300D1399Y Does Not Affect TFAP2A DNA Binding Activity (EMSA with nuclear extracts prepared from Hep3B cells) or CITED2 Nuclear Localization (immunolocalization in Hep3B cells). The HAT-deficient p300 mutant (p300D1399Y) does not interact with TFAP2A (co-IP of overexpressed proteins in Hep3B cells).

Elodie Portales-Casamar
Additional Reviewer Comments:
Reviewer: Elodie Portales-Casamar

p300 is a well characterized coactivator, binding to many TF and activating transcription through its HAT activity.


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Facts about Ep300 328572RDF feed
Gene description e1a binding protein p300  +
Gene id 328,572  +
Gene judgment TF Gene  +
Gene symbol ep300  +, A430090G16  +, A730011L11  +, and p300  +
Gene taxonomy Transcription Factor Binding: tf co-factor binding  +
Homolog cluster 12,914  +
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